Striker Andrea Belotti and midfielders Marco Verratti and Lorenzo Pellegrini are excluded from the Italian national team's roster due to injuries, the Italian soccer federation said in a statement on Monday.
Coach Gian Piero Ventura decided to replace the three injured players with Roberto Inglese, Roberto Gagliardini and Bryan Cristante for the upcoming qualifiers against Macedonia and Albania.
Ventura also called up midfielder Nicolo Barella, 20.
Belotti underwent tests that detected problems with his right knee, while both Verratti and Pellegrini suffered with physical problems.
The Italian national team is to perform a training session on Monday afternoon in Italy.
Italy is set to host Macedonia on Oct. 6 and will travel to face Albania three days later.
The 26-player squad of Italy includes:
Goalkeepers: Gianluigi Buffon (Juventus), Gianluigi Donnarumma (Milan), Mattia Perin (Genoa).
Defenders: Davide Astori (Fiorentina), Andrea Barzagli (Juventus), Leonardo Bonucci (Milan), Giorgio Chiellini (Juventus), Danilo D'Ambrosio (Inter), Matteo Darmian (Manchester United), Daniele Rugani (Juventus), Leonardo Spinazzola (Atalanta), Davide Zappacosta (Chelsea);
Midfielders: Nicolo Barella (Cagliari), Bryan Cristante (Atalanta), Daniele De Rossi (Roma), Roberto Gagliardini (Inter), Marco Parolo (Lazio) Federico Bernardeschi (Juventus), Antonio Candreva (Inter), Stephan El Shaarawy (Roma), Lorenzo Insigne (Napoli), Simone Verdi (Bologna);
Forwards: Eder Citadin Martins (Inter), Manolo Gabbiadini (Southampton), Ciro Immobile (Lazio), Roberto Inglese (Chievo).